Answer questions and please give sources u used!!!!

Answer Questions And Please Give Sources U Used!!!!

Answers

Answer 1

Answer:

1. The Qing dynasty was the last imperial dynasty of China, ruling from 1644 to 1912.

2. The Qing dynasty was founded by the Manchu people, who originally lived in the northeastern part of China.

3. The Qing dynasty saw significant expansion of the Chinese empire, including the incorporation of Tibet, Xinjiang, and Mongolia into the empire.

4. The Qing dynasty implemented a series of economic and social reforms, including the introduction of a new currency and the establishment of a nationwide system of education.

5. The Qing dynasty had significant interactions with other civilizations, including Europe, Japan, and the United States. This included trade, diplomatic exchanges, and military conflicts.

6. One of the most significant events in the history of the Qing dynasty was the First Opium War, in which China was defeated by Britain and forced to open its ports to foreign trade.

7. The Qing dynasty left a lasting legacy on China, including the development of a centralized government and the spread of Han Chinese culture throughout the empire.

8. Some of the most significant political and social developments during the Qing dynasty included the introduction of a civil service examination system, the establishment of a centralized bureaucracy, and the introduction of western ideas and technologies.

Explanation:


Related Questions

Which sentence has the correct comma placement

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where is the sentence? What’s the question? I think you forget to actually input the question.

Can you help me with English homework

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Sure I don’t see a picture but you can make one so that I can help you out

What is a good conclusion on why teachers are important??( i need 1 paragraph with 5 sentences in it)

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In conclusion, we need teachers because they help us in many different ways. They show us the right way when we do something wrong, they try to make us the best versions of our selves, they care about our education and how important having an education is, they try to help us when we’re struggling in both school and life, in general, they’re important because we as students need someone to help us through hard times, and that’s what teachers do.

pls answer the below things

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Answer:

Explanation:

understandable

Answer:

2 ate

3 she is agood person

4 is

5 she is going

6 i eat my

7

Problem: What type of sensory word
would you not use in a narrative?
a. Loud
b. Smelly
c. Hopeful
d. Beautiful

Answers

In a narrative, you would not use a sensory word like "hopeful" because it does not describe a physical sensation that can be sensed by one of the five senses (sight, hearing, touch, taste, and smell). Sensory words are words that describe a physical sensation, so "hopeful" is not a sensory word. Instead, you could use words like "loud," "smelly," or "beautiful" to describe a physical sensation in a narrative.

Respond to the following question using PEELS. You must use evidence from the book to support your claim.

What revelation does Horatio have about Gilbert and Roscoe and what they are really doing with Hamilton?

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The revelation that Horatio has about Gilbert and Roscoe is to swear secrecy pertaining to the ghost and Hamlet's "antic disposition."

What is Horatio's purpose at the end of the play?

At the end of the play, when Hamlet is killed in his fight with Laertes, Horatio, in his grief, offers to kill himself with his own sword. It is Hamlet's dying request that Horatio tell Hamlet's story, and let the truth of it be known, that keeps him from doing so.

According to the context of this question, Hamlet explains to Horatio how the play will help prove the Ghost's honesty and reveal Claudius' perfidy. He asks Horatio to watch the King and note his reaction to a specific speech in Murder of Gonzago.

Hamilton's view on slavery greatly affects their ideology in the context of the ghost story by Horatio.

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For Daisy was young and her artificial world was redolent of orchids and pleasant, cheerful snobbery and orchestras which set the rhythm of the year, summing up the sadness and suggestiveness of life in new tunes. (Fitzgerald p152)Character DevelopmentTheme Development

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The quote from F. Scott Fitzgerald’s The Great Gatsby speaks to the character of Daisy and her artificial world of privilege and beauty. Daisy is young and unaware of the realities of life, instead living in her own dreamy world of orchids and music. This quote speaks to Daisy’s character development and the themes of sadness and suggestiveness of life.

Throughout The Great Gatsby, Daisy is presented as a young, naive woman. She is portrayed as a kind of idealized version of a young woman, living in a world of her own making. Daisy is unaware of the realities of life, instead living in a world of luxury and pleasure. She is surrounded by beauty and music, with everything set in perfect harmony. This is symbolic of Daisy’s character development throughout the novel. She is presented as an idealized version of a young woman, unaware of the realities of life.

The quote also speaks to the themes of sadness and suggestiveness of life. Daisy is living in her own world, unaware of the sadness and suggestiveness of life. This is symbolic of the theme of the novel, which is that life is not as perfect as it appears to be. The music and beauty of Daisy’s world serves as a reminder of the harsh realities of life. It is a reminder of the sadness and suggestiveness of life that Daisy is not aware of.

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Who was elected? Our new color

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Parents that have to work multiple jobs to pay rent cannot take weekends off to drive to tournaments.

What are the actual face of people?

The only people on the fields are the people with the means to get there. Parents that have to work multiple jobs to pay rent cannot take weekends off to drive to tournaments.

Parents who are caring for their own elderly parents cannot leave town overnight to sleep in hotels away from those they're responsible for. Parents who cannot afford a car, who live paycheck to paycheck, are not the parents with children on those fields.

Therefore, Parents that have to work multiple jobs to pay rent cannot take weekends off to drive to tournaments.

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how does Twain show awareness of his audience?
He uses a quotation to establish his credibility
He narrates the scene to introduce the tops
He uses imagery to rpovide context
He interprets the quotation

Answers

Mark Twain aimed for a large audience. Through membership sales and the monthly press, he was able to connect with readers both domestically and overseas, and this widespread popularity was ideal for him.

Except for The Prince and the Pauper and Joan of Arc, every book Mark Twain ever published was written with the intention of being sold, according to Kenneth Andrews. Mark Twain frequently informs his contacts in his letters how many editions of his books have indeed been sold and the amount of money he has made. This implies that he wrote with audience appeal in mind. The letters written by Twain further demonstrate his constant concern for the attendance at his lectures. They demonstrate how frequently he was aware of the audience.

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the committee is interested _____your thoughts

Answers

Answer:

he committee is interested in hearing your thoughts.

Explanation:

hope this helped:)

brainliest for the brains:)

I ___ to the gym last night.
a. gone
b. was
c go
d went

Answers

Answer:

d) went

Explanation:

In this sentence, the subject is "I," which is in the first person singular. The verb "went" is in the past tense and is correctly conjugated to match the subject. "Gone" is the past participle of the verb "go," and is used in conjunction with an auxiliary verb (such as "have" or "had") to form verb tenses such as the present perfect or past perfect. "Was" is the past tense of the verb "be," and is not appropriate for this sentence. "Go" is the present tense of the verb "go," and is not appropriate for this sentence as it is written in the past tense.

Answer:d

Explanation:it is d because all the other answer don't make sense int the sentence

My questions weren't answered. (Change into active voice)​

Answers

Answer:

no question Given here give question

Have you ever misplaced your purse or wallet? This may be a thing of the past since some companies are developing personal identification purchasing capability using ___ software.

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This may be a thing of the past since some companies are developing personal identification purchasing capability using preventative measures software.

Never provide personal information to a stranger as a protective step against ID fraud or theft.Currently, identity theft happens when a person or hacker with nefarious motives seeks to access another person's private or secret information. And the best method to stop this hacking is to make sure.

That all of your personal data is maintained in a secure location with the required security measures in place, as well as being extremely cautious about who you share your personal information with.Identity theft happens when an individual with malicious intent obtains your personal information. With this information they can open accounts in your name, steal your money, ask for loans etc.

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What does a good leader act
like?

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Answer:

Good leaders possess self-awareness, garner credibility, focus on relationship-building, have a bias for action, exhibit humility, empower others, stay authentic, present themselves as constant

and consistent, become role models and are fully present.

A good leader should have integrity, self-awareness, courage, respect, empathy, and gratitude. T

Explanation:

i hope i helped

Question 11 (2.5 points)
HIV medications can be used to prevent HIV infection.
1) True
2) False

Answers

The answer is true. Have a nice day

Select the thesis statements that are most unified.

Teen magazines are an inexpensive source of well-written fiction and nonfiction.
Teen magazines are an inexpensive source of well-written fiction.
Teen magazines are an inexpensive source of well-written nonfiction.
Teen magazines are an inexpensive source of reading materials.

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Teen magazines are an inexpensive source of well-written fiction. that the thesis statements that are most unified.

The thesis statement unites what?

Specific: Each thesis statement in a given topic is narrowly focused on a single issue. A question can be used to express a problem. Unified: The entire thesis phrase must center around a single main point.

The primary idea in a piece of writing, or the thesis sentence, reflects the writer's viewpoint on the subject, as well as their intent and attitude. The sentences that follow are all related to this main idea. A thesis statement could describe how the writing is structured.

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2. It has been a tiring day ____
a. . (full stop)
b. ! (exclamation)
c. ? (interrogation / question mark

Answers

Answer:

a. Full stop

Explanation:

the reason why we put full stop is to end his sentence

NEED HELP ASAP!!!
Which phrase describes the mood of a literary text?
A.the surface-level events or ideas in a text
B. the attitude of a text toward its subject
C. the emotions a text may create for a reader
D. the time, place, and environment of a text

Answers

In my thinking, c is the correct answer.

The emotions a text may create for a reader

I think the answer is C

the purpose of this passage is to A:tell the reeaders about the type of animals that spread disease

Answers

The purpose of this passage is option C) persuade readers to purchase an all-natural pest prevention product.

What are author's purposes?

The goal or objective of a writer is their motivation for writing. An author may want to mock a condition, inform the reader, persuade the reader, or amuse the reader.

This passage's primary goal is to accomplish C), persuade readers to buy an organic pest control product. That objective receives the most of its paragraphs.

Therefore, This passage begins by educating readers about the kinds of animals that spread sickness, but it merely serves as the prelude to its campaign to get readers to purchase the goods. Educates readers on the risks associated with commercial chemicals, but solely in order to highlight its all-natural cures. Hence option C: is correct.

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See full question below

As the weather becomes warmer and people travel outside more often, daring pests creep indoors to explore kitchens and pantries. It is important to know that insects and mice can contribute to the spread of germs and diseases. At the very least, these pests can damage food supplies or cause itchy bug bites. From the largest mice to the tiniest fleas, these creatures can turn a peaceful home into a crawling nightmare!

2Many people, however, hesitate to use commercial sprays near children and pets or in places where they store food. The harsh chemicals in these sprays can cause serious injuries if they aren’t used properly. Luckily, there is now another choice for people who want to avoid these dangerous chemicals. The experts at Growing Garden Supply are happy to help you discover all-natural ways of keeping these creatures outdoors, where they belong.

3This week, pots of peppermint and lavender plants are on sale. These herbs not only let off fragrant scents, but they also can be used to keep pests out of your home. Place a pot of lavender in the kitchen to ward off mice and use mint as an all-purpose pest remover. The scent of crushed mint leaves is unpleasant for mice, ants, and fleas. Sprinkle a few along the edge of your home to discourage pests from entering. Happily, mint leaves are completely safe for humans to eat. Try some in a cup of tea, or use them to add flavor to baked goods.

4After seeing the great results from the mint and lavender plants, why not start an entire garden of plants that are unappealing to pests? Growing Garden Supply has an excellent selection of bitter cucumber seeds and garlic bulbs. Slices of cucumbers and garlic can help chase away ugly roaches and mosquitoes, and the leftovers taste wonderful in summer salads and pasta dishes.

5People who are eager for an immediate fix to their pest problems might prefer a liquid spray. Fortunately, we have many plant oils and spray bottles in stock! Some of these essential oils can be combined for an even greater effect. Remember, essential oils cannot be stored in plastic or metal for long periods—the oils will eat away at the container. Instead, check out Growing Garden Supply’s impressive collection of beautiful glass jars, available in a variety of shapes and sizes. This will solve all of your oil storage problems.

6Once the house is free of pests, consider natural ways of keeping wildlife from attacking your garden and yard. Choosing the right plants to grow together can be a natural way of keeping hungry caterpillars and rabbits away. Ask our specially trained employees for more helpful suggestions on pest prevention both in and outside the home.

The purpose of this passage is to

A) inform readers of the dangers of commercial chemicals.

B) tell readers about the types of animals that spread disease.

C) persuade readers to purchase an all-natural pest prevention product.

D) show readers why it is important to follow directions when using sprays.

hmh american history module the english colonies 3 assessment answers 'b. draw conclusions why was maryland's toleration act of 1649 important?'

Answers

hmh american historic Communities On December 6, 1606, three ships departed from England in search of a new home in Virginia. illness and disease The germs, bacteria, and ailments that existed on this new continent.

Were unknown to the explorer the largest problem colonists in Virginia faced when they first arrived was a lack of food because the soil was hard to cultivate and the Native Americans might be hostile. However, once they had enough tobacco growing, they were able to trade efficiently.

In an effort to find gold, the London-based Virginia Company dispatched ships to sea in 1606. The English settlers who founded Jamestown did not find gold; rather, they endured starvation, disease, and severe misery. Only 38 of the 144 colonists that arrived in Jamestown survived the first year.

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What seems to be the poet's attitude about war? In your opinion, is war usually a worthy
endeavor for humankind? Use examples from the text as well as your own life experiences. Don't
forget to use the S-Summarizing sentence at the end. Please help asap

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In the poem 'There Will Come Soft Rains', the poet's attitude about war is that -War is a necessary evil that will eventually bring peace.

The poem  'There Will Come Soft Rains' is stunning and full of vivid imagery. Teasdale discusses the effect or lack there of—that humanity actually has on the natural world in this passage. Despite the fact that we are undoubtedly very destructive, our interactions with other living things are so one-sided that they would not even notice if we suddenly vanished from the planet. Teasdale makes references to war while talking about the natural world. The 1918 German Spring Offensive in the First World War inspired the song "There Will Come Soft Rains." It's likely that Teasdale also drew inspiration from the concurrent 1918 flu pandemic.

How do you summarize the poem 'There Will Come Soft Rains'?

The speaker of the poem begins by listing several peaceful scenes. There are frogs croaking in water at night and birds circling, serenading out their "shimmering sounds." The world's creatures, trees, and wind are all in harmony and are satisfied with one another.

The poem's second half explains how nature and "Spring" wouldn't notice if all people were at war. They wouldn't be at all affected by it. Additionally, considering how little humans work perfectly into their world, they would not care if everyone on the planet vanished.

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What literary device is most prominent in this excerpt from Sandburg's poem, "Grass"?
"Pile the bodies high at Austerlitz and Waterloo."
alliteration

simile
enjambment
allusion

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The literary device that is most prominent in this excerpt from Sandburg's poem "Grass" is option A:  alliteration.

What is the alliteration about?

Alliteration is the repetition of the same sound or group of sounds at the beginning of two or more words in a phrase or line of poetry.

In this case, the repetition of the sound "P" at the beginning of the words "Pile" and "bodies" is an example of alliteration.

Therefore, one can say that alliteration is often used in poetry as a way to create a sense of unity and flow within a poem, and to draw the reader's attention to certain words or sounds.

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Identify if each of the given sentences is a simple, compound, or complex sentence.

1. l enjoyed the play, but I didn't like the end.

2. Jean was excited because her poem was greatly appreciated by all.

3. How fabulous our Christmas tree looks with all the decorations!

4. Even if our team wins this match, we may not make it to the final.

5. Dr. Mark prescribed some pills, and he told the patient to have a week's rest



PLEASE IT'S DUE TOMORROW MORNING!!!!!?!?!?!

Answers

A simple phrase just has one clause. A complex sentence consists of one independent clause one and or more dependent (subordinate) clauses. A compound sentence is made up of a number of linked but independent clauses.

1. Complex Sentence

2. Simple Sentence

3. Simple Sentence

4. Complex Sentence

5. Compound Sentence

Three things make up a basic sentence: the subject, the verb, and the finished idea.

A complex sentence consists of one independent clause as well as at least one clause. When you wish to develop or modify the main idea of your statement, it works best.

Two separate clauses are joined together in a compound sentence with a conjunction, most typically and or but. When two or more separate, connected sentences are blended into one, they work best.

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critique the theories explaining division of household labor. Do you think these theories are true and accurate or false. Explain your answer. What advice would you give men regarding division of labor at home and childcare? What advice would you give women on the same issue? At what age would the children help with household chores, if any? What would children do?

Answers

The division of domestic labour is the distribution of responsibilities involved in maintaining a family functioning efficiently in the home, including chores like cleaning, cooking, laundry, and childcare.

What is division of household labor?Division of domestic labor refers to the distribution of responsibilities involved in maintaining a family functioning normally in the home, including chores like cleaning, cooking, laundry, and childcare. In the past, males have typically provided for their families financially while women have historically specialized in household chores, performing between three-quarters and two-thirds of the regular household work. Housework is unpaid, devalued, and disempowering while earning a living is compensated, valued, and empowering. Women who perform the majority of the housework are also negatively impacted by this when it comes to their ability to participate in the paid labor force.While women's participation in the labour force increased during the course of the 20th century, the amount of housework they contributed decreased, and males contributed more to home duties even while women remained the majority of the workforce. Popular theoretical explanations for the gender gap in household contributions at the family level include time availability, relative resources, and gender ideology, while more recent theoretical perspectives look at work-family and gender policy at the national level as contexts in which the family distribution of household labour is embedded.

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A A
25. According to the article, how could smart cities
worsen existing problems?
OA. Smart cities are more likely to attract large
companies and investors, indirectly harming
the economies of traditional cities.
OB. Certain areas of smart cities could be made
more advanced than others, creating greater
inequality between citizens.
O C. Large cities are more likely to become smart
cities, creating greater inequality between
cities of varying sizes.
O D. Citizens who live centrally in smart cities are
more likely to benefit than those who live on
the outskirts.

Answers

Answer:

OB. Certain areas of smart cities could be made

more advanced than others, creating greater

inequality between citizens.

Explanation:

Final answer:

Smart cities could worsen existing problems by attracting large companies and investors away from traditional cities, creating intra-city inequalities due to uneven distribution of advanced technologies, exacerbating inequalities between cities of different sizes, and benefiting centrally-located residents at the cost of those living on the outskirts.

Explanation:

The article seems to imply that the development of smart cities could potentially exacerbate existing problems in a variety of ways. One possible downside is that these highly-advanced cities could attract large companies and investors, which could disadvantage traditional cities by draining their economic opportunities (Option A).

Smart cities could also breed inequality within their own boundaries since certain areas could be more technologically advanced than others, leading to an unbalanced distribution of resources and services (Option B). Additionally, larger cities are more likely to be transformed into smart cities, which can compound inequalities between cities of different sizes (Option C). Lastly, those who live centrally in smart cities could reap more benefits than those on the outskirts, adding another layer of inequality (Option D).

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Please read the excerpts from"Civil Disobedience" by Henry David Thoreau. What is his central idea? Identify his central idea. Please support your answer with THREE pieces of textual evidence and analysis.

Its a portfolio and if you completed it can you please help!!!

Answers

To gain the right to levy taxes on its inhabitants, according to Thoreau, the government must put an end to its unfair practices. He went on to say that as so long as the government did wrong things, moral people had to decide to either pay their taxes or not and rebel against it.

Civil disobedience is the peaceful rejection of a rule of law, regulation, or authority that is deemed unfair. Therefore, civil disobedience is a nonviolent form of opposition.

The main takeaway from Thoreau's book Walden is to live simply, on your own, and sensibly. He advises people to attempt to live a free and uncommitted life, free from the exchange economy and modern labor, and other things that make life too complicated. He also stresses the value of having as close of a direct relationship with Nature as possible.

Thoreau observes that physicians frequently advise patients to change their surroundings when they are ill, but he subtly mocks this notion by asserting that "the cosmos is bigger than our conceptions of it." He contends that rather than a change in the environment, perhaps a change in the soul is required.

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Read the passage from act 2, scene 3 of The Tragedy of Macbeth.

[Porter.] Lechery, sir, it provokes, and unprovokes; it provokes the desire, but it takes away the performance. Therefore much drink may be said to be an equivocator with lechery; it makes him, and it mars him; it sets him on, and it takes him off; it persuades him, and disheartens him; makes him stand to, and not stand to; in conclusion, equivocates him in a sleep, and, giving him the lie, leaves him.

What is the effect of the antithesis in the porter’s speech?

It explains why some people use alcohol.
It contrasts the porter’s demeanor to that of other servants.
It describes the results of becoming intoxicated.
It compares the porter to the other revelers in Macbeth’s household.

Answers

The effect of the antithesis in porter’s speech It compares porter to the other revelers in Macbeth’s household.

What is the antithesis?

When two opposites are put together for contrasting effects, an antithesis is employed in writing or speech as a proposition that contrasts with or reverses some previously mentioned concept. This is based on the term or phrase that makes sense.

There are two reasons why Porter is significant in Macbeth. Porter's primary purpose is to provide humorous relief by releasing the tension that has built up in the audience as a result of King Duncan's murder. By implying that the gates to Macbeth's house are analogous to the gates of hell, Porter also serves a thematic purpose.

Therefore, Thus option (D) is correct.

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write the summary of the poem all the worlds a stage​

Answers

Explanation:   Summary

In the poem, Shakespeare conveys the notion that human life is not truly real. The things we see and hear are not true. Human life is a stage production. The infant in arms, the schoolboy, the lover, the soldier, the just, the retired man, and the worn-out senior, sliding back into dissolution are just a few of the ages Shakespeare depicts in this play as he tracks the progression of human life through the renowned seven ages. The entire planet is a stage. We are only pretenders. We get onto the stage and exit it once more. In his lifetime, a guy takes on numerous roles. He initially assumes the role of the baby, wailing and spitting milk into the nurse's arms.

Then he assumes the role of a schoolboy who is reluctant to attend class. He trudges along at the snail's pace while wearing his morning smile. The lover then appears. He writes pathetic verses to his mistress while sighing like a fire. He portrays him as a soldier. It is filled with all of the harsh swear words. He has a gorgeous beard on. He is overly quick, sensitive, and hasty when arguing. He is prepared to give his life in exchange for hollow glory. He will then assume the position of judge. He has a manly tummy and stern eyes. He has great wisdom.

Shakespeare then elaborates on his advanced age. It's quite humorous. The elderly man is wearing glasses and slippers. His once-manly voice now has the shrill tone of a young child. The second child plays the final role. There is so much forgetfulness there. It lacks everything—teeth, eyes, taste, and everything else.

The poem "All the World Is a Stage" is appreciated.

William Shakespeare, a well-known English poet and playwright, wrote the poem "All the World is a Stage." This poem doesn't follow any particular rhyme system, but it maintains a constant rhythm with five beats between each line. The poem is a blank verse because the rhythm is constant.

Alliteration, consonance, hyperbole, inversion, metaphor, metonymy, onomatopoeia, repetition, simile, and transferred epithet are some of the figures of speech utilized in the poem. The poem uses similes throughout, such as "Then a soldier full of weird oaths and bearded like a part," which compares a soldier's beard to a leopard. Essentially, the poem is a metaphor for life. Each man must play a variety of roles.

Each man has a unique set of responsibilities in his life. It proves that life is structured in a certain order, with each person going through different stages as they spend their lives. It exemplifies the idea of life's continual transformation.

17
Select the correct answer.
What is the most important reason for citing a source when quoting it directly?
A. to give credit to the source
B to make your work seem complete
C. to add more information to your work
D. to pick ideas from different sources
Reset
Nextlin

Answers

Answer: A.

Explanation:

A - to give credit to your source - is the best answer and makes the most sense.

When you cite a source, you give credit to it. For example, if you said a quote and didn't cite your source, you would be stealing their words. You must always cite to avoid plagiarism

Write a paragraph comparing and contrasting Citra Terranova with another character from the book Scythe. Be sure to write at least 5 sentences, reference the text with appropriate MLA citations, explain each character's morals/beliefs, and include both similarities and differences.

Answers

In the book Scythe, Citra Terranova is a young scythe apprentice who is paired with the experienced scythe Faraday. Citra is independent and resourceful, and she is determined to do whatever it takes to become a successful scythe. In contrast, another character in the book, Rowan Damisch, is also a scythe apprentice, but he is much more hesitant and unsure of himself. Whereas Citra is confident and willing to take risks, Rowan is more hesitant and cautious. However, both Citra and Rowan share a strong sense of justice and morality, and they both struggle with the difficult choices that come with being a scythe.

I'm sorry, I don't think I met all of the requirements (don't MLA citations for example) but I tried my best

Other Questions
Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (KD) Distance Migrated (mm) 37 23 25 30 20 35 15 42 51.5 10 Distance Migrated (mm) vs. Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (kD) 40 30 Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight 20 10 25 30 35 40 45 50 Distance Migrated (mm) STUDENT MANUAL POST-LAB ACTIVITY Procedures Generate a standard curve to calculate protein sizes The different protein bands in your gel can be defined by their different molecular masses. Indeed many proteins are named for their molecular weights. For example p53, a protein implicated in tumor progression is 53 KD in size. To determine the molecular masses of the proteins, a standard curve is created plotting the known molecular masses of the proteins in the Precision Plus Protein Kaleidoscope prestained standards against the distance they have migrated down the gel from the base of the well. A 15% polyacrylamide gel is designed to separate small proteins-proteins less than 40 KD. Your gel analysis will concentrate on this size range. Note: If a different percentage acrylamide gel or an agarose gel has been run, analyze the section of the gel that has the best separation 1. As shown in the figure below draw a line between the 37 and 25 KD bands of the prestained standards. Your gel analysis will be restricted to the proteins below this line. Stenders Actin & Myosin 4 Measure distance from base of wells to the base of the bands 250 150 100 75 50 37 25 20 Measure Standard bands between 37 and 10 kD 15 Measure fish protein bands between 30 and 10 KD 10 - Fig. 18. Image of fish muscle proteins separated by SDS-PAGE and stained with Bio-Safe Coomassie stain. Lines illustrate measurement of bands for constructing the standard curve. STUDENT MANUAL POST-LAB ACTIVITY Define the characteristics (proteins) of the different fish 4. For each fish sample that has been analyzed, determine the molecular masses of the proteins below the 25-37 KD line. Measure the distance each band has migrated from the base of its well. Find that distance on the x-axis of the standard curve. Draw a line up from the x-axis to the curve. Read across to the y-axis to determine the molecular mass. Alternatively, use graphing software to generate the standard curve. Make a line of best fit (or trend line) through the points and formulate an equation to calculate the mass of the unknown proteins on the gel. 5. Enter this data into a table with the molecular masses of the proteins for each fish (see example below). Fish Species A Distance Molecular Migrated Mass (mm) (KD) 25 32.5 26.5 31 29 28.6 36 21.7 21.2 36.5 39 18.8 44 13.9 52 6 Determine which fish have each characteristic (protein) 6. Make a table with a row for every band size you have recorded for all your fish samples and a column for each type of fish on your gel. Then make a mark in each cell of the table where the fish has that size band (see example below). -LAB ACTIVITY ENT MANUAL Species A Species B Species c Species D Species E X X X X 27.5 X X X X X X 33 X X X X Characteristic Protein Distance Molecular Migrated Mass (mm) (kDa) 25 32.5 26 31.5 26.5 31.0 30.0 28.5 29.1 29 28.6 30 27.6 30.5 27.1 32 25.6 24.7 34.5 23.2 35.5 22.2 36 21.7 36.5 21.2 37 20.7 37.5 20.2 38 19.7 38.5 19.3 39 18.8 39.5 18.3 40.5 17.3 41 16.8 41.5 16.3 42 15.8 43 14.8 44 13.9 45 12.9 46 11.9 46.5 11.4 47 10.9 47.5 10.4 51.5 6.5 52 COUNT X X X X X X X X 6.0 8 10 13 10 12 Find the number of characteristics shared by each of the fish 7. In the table below both the row and column headings are the types of fish. From the table above, separately compare the number of bands (X's) in common with every other fish sample from your gel and put those numbers into the table below, such that each fish is individually compared with every other fish. In this example, species A and B have just 2 bands in common while species B and C have 10 bands in common. Your table will be the basis for drawing your cladogram. Species A Species B Species c Species D Species E Species A 8 2 2 2 Species B 10 10 5 3 Species C 13 5 Species D 2 Species E Is direct variation the same as slope? Which cells are diploid in meiosis? What are 4 values and 12 principles of Agile? How do you solve equations on a calculator? More than one of the following may be correct. Select all correct choices. A sharp increase in capillary hydrostatic pressure would directly cause lewis structure for caffeine I need help with this Express 2678 as a product of prime numbers On January 1, 2024, Canseco Plumbing Fixtures purchased equipment for$52,000. Residual value at the end of an estimated 7 year service life is expected to be$24,000. The company expects the equipment to operate for 15,500 hours. Required: a. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on January 1,2024 b. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on March 31, 2024 Complete this question by entering your answers in the tabs below. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on January 1, 2024. On January 1, 2024, Canseco Plumbing Fixtures purchased equipment for$52,000. Residual value at the end of an estimated 7 year service life is expected to be$24,000. The company expects the equipment to operate for 15,500 hours. Required: a. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on January 1, 2024. b. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on March 31, 2024 Complete this question by entering your answers in the tabs below. Calculate depreciation expense for 2024 and 2025 using sum-of-the-years'-digits, assuming the equipment was purchased on March 31, 2024. What was a vindication of the rights of woman a response to? What is the first thing an entrepreneur do first when starting a new business? eccles incorporated, a zero growth firm, has an expected ebit of $100,000 and a corporate tax rate of 25%. eccles uses $500,000 of 12.0% debt, and the cost of equity to an unlevered firm in the same risk class is 16.0%. refer to the data for eccles incorporated. what is the value of the firm according to mm with corporate taxes? a. $653,125 b. $593,750 c. $534,375 d. $718,438 e. $480,938 What is the measure of ZE, in degrees? D 48 5 O A. 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